The effect of mephedrone on human neuroblastoma and astrocytoma cells

被引：1

作者：

Alanazi, Ibrahim M. ^{[1
]}

Alzahrani, Abdullah R. ^{[1
]}

Alsaad, Mohammad A. ^{[2
]}

Moqeem, Abdulaziz L. ^{[3
]}

Hamdi, Abdulmohsen M. ^{[3
]}

Taher, Mohiuddin M. ^{[4
,5
]}

Watson, David G. ^{[6
]}

Grant, M. Helen ^{[7
]}

机构：

[1] Umm Al Qura Univ, Coll Pharm, Dept Pharmacol & Toxicol, POB 13578, Al Abidiyah 21955, Makkah, Saudi Arabia

[2] Umm AL Qura Univ, Coll Med, Dept Parasitol, POB 13578, Al Abidiyah 21955, Makkah, Saudi Arabia

[3] Minist Hlth, Home Hlth Care, Directorate Hlth Affairs Jeddah, POB 11176, Ryiadh, Saudi Arabia

[4] Umm Al Qura Univ, Sci & Technol Unit, Deanship Sci Res, Mecca, Saudi Arabia

[5] Coll Med, Umm Al Qura Univ, Dept Med Genet, Mecca, Saudi Arabia

[6] Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, Glasgow G4 0RE, Scotland

[7] Univ Strathclyde, Dept Biomed Engn, Glasgow G4 0NW, Scotland

来源：

SAUDI PHARMACEUTICAL JOURNAL | 2024年 / 32卷 / 04期

关键词：

Mephedrone; Glutathione; Metabolomics; LC-MS/MS; 4-METHYLMETHCATHINONE MEPHEDRONE; METHCATHINONE ANALOGS; LIQUID-CHROMATOGRAPHY; OXIDATIVE STRESS; NERVE-ENDINGS; FATTY-ACIDS; RAT-BRAIN; IN-VITRO; STABILITY; AMPHETAMINE;

D O I：

10.1016/j.jsps.2024.102011

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Mephedrone is an illegal drug that is used recreationally. Few studies have been conducted to investigate the mechanisms by which mephedrone is harming cells. In this research, we investigated the effect of mephedrone using toxicology coupled with LC-MS/MS based metabolomics in the two CNS derived cell lines. Methods of assessment such as neutral red (NR) assay, dimethylthiazolyl diphenyltetrazolium bromide (MTT), lactose dehydrogenase (LDH) measurement, and morphology were performed to identify the effect on cell viability and to identify the best concentration to be used in a metabolomics study. A concentration of 100 mu M of mephedrone was used in the metabolomic experiment because at this concentration mephedrone had induced several intracellular changes. Although there no clear indicators of cellular damage caused by mephedrone. In astrocytes there was a clear indication that cell membrane function might be impaired by depletion of ether lipids.

引用

页数：13

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