Biosynthesis of CuO@Au NPs and Its Formulated into Biopolymers Carboxymethyl Cellulose and Chitosan: Characterizations, Antimicrobial, Anticancer and Antioxidant Properties

Nanoparticles (NPs) have experienced an explosive global growth as a key byproduct of nanotechnologies over the last ten years due to their remarkable physiochemical qualities. Bimetallic nanoparticles are a promising combination of two kinds of nanoparticles that often display synergetic behaviors. In the current study, a technology that is beneficial to the environment was used to biosynthesize copper oxide and gold nanoparticles (CuO@Au NPs) utilizing a fungus isolate. Using carboxymethylcellulose (CMC) and chitosan (Ch), formed nanoparticles were turned into biopolymers (nanocomposite) using a green method. CuO@Au NPs and nanocomposite qualities were investigated using physical and chemical methods including FITR and XRD, as well as topographical methods like FESEM and HRTEM. About 20 nm-diameter bimetallic NPs were identified in the nanocomposite, which was also determined to have verified nanostructures. CuO@Au NPs exhibited less antimicrobial activities with inhibition zones of 15, 27, 8, and 25 mm than nanocomposite with inhibition zones of 16, 26, 29, and 14 mm against E. coli, E. faecalis, C. albicans, A. flavus, and M. circinelloid respectively. CuO@Au NPs reflected more MIC value against S. aureus, (62.5 mu g/mL), S. typhi (62.5 mu g/mL), E. faecalis (31.25 mu g/mL) than MIC value of nanocomposite against the same bacteria. Moreover, the MBC values of CuO@Au NPs were more (ranged from 31.25 to 125 mu g/mL) than that of nanocomposite (ranged from 15.62 to 62.5 mu g/mL) against tested bacteria. DPPH scavenging % indicated that nanocomposite was promising than CuO@Au NPs with IC50 value of 116.87 mu g/mL and 173.34 mu g/mL, respectively. Effect of CuO@Au NPs and nanocomposite on Wi38 reflected high IC50 of 585.15 +/- 1.58 mu g/mL and 587.14 +/- 0.29 mu g/mL, respectively against Wi38 cells. However, the recorded IC50 values of CuO@Au NPs were less (166.56 +/- 2.91 mu g/mL) than IC50 (181.8 +/- 4.27 mu g/mL) of the nanocomposite against MCF-7 cells.