Electrochemical DNA-based sensors for measuring cell-generated forces

被引：6

作者：

Tabrizi, Mahmoud Amouzadeh ^{[1
]}

Bhattacharyya, Priyanka ^{[1
]}

Zheng, Ru ^{[1
]}

You, Mingxu ^{[1
,2
]}

机构：

[1] Univ Massachusetts Amherst, Dept Chem, 710 N Pleasant St, Amherst, MA 01003 USA

[2] Univ Massachusetts Amherst, Mol & Cellular Biol Grad Program, 710 N Pleasant St, Amherst, MA 01003 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2024年 / 253卷

关键词：

Cellular forces; DNA probe; Electrochemical sensor; Hairpin DNA; Tension gauge tether; INTEGRIN; INTERFACE; CANCER;

D O I：

10.1016/j.bios.2024.116185

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Mechanical forces play an important role in cellular communication and signaling. We developed in this study novel electrochemical DNA -based force sensors for measuring cell-generated adhesion forces. Two types of DNA probes, i.e., tension gauge tether and DNA hairpin, were constructed on the surface of a smartphone-based electrochemical device to detect piconewton-scale cellular forces at tunable levels. Upon experiencing cellular tension, the unfolding of DNA probes induces the separation of redox reporters from the surface of the electrode, which results in detectable electrochemical signals. Using integrin-mediated cell adhesion as an example, our results indicated that these electrochemical sensors can be used for highly sensitive, robust, simple, and portable measurements of cell-generated forces.

引用

页数：8

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