Effects of Oxidized Low Density Lipoprotein onthe Expression and Function of ABCA1 in Macrophages

被引：0

作者：

李映红

毕昊

吴凡

宗义强

王燕

屈伸

机构：

[1] Department of Biochemistry & Molecular Biology

[2] Tongji Medical College

[3] Huazhong University of Science and Technology

[4] Wuhan

[5] China

来源：

华中科技大学学报(医学英德文版) | 2005年 / 02期

关键词：

ATP binding cassette transporter A1; cholesterol efflux; low density lipoprotein; gene expression regulation;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

Summary:In the present study, we examined the regulation of the expression and function of ABCA1 by modified LDL (ox-LDL) in vitro. After incubation with apoA-I for 24 h, RAW264.7 cells effluxed 37.65 % cholesterol loaded by acetyl LDL (ac-LDL), and 9.78 % cholesterol in ox-LDL group. The level of ABCA1 mRNA increased about three times either when cells were incubated with 100 μg /mL ac-LDL or with 100 μg /mL ox-LDL. However, the level of ABCA1 protein rose by 1.57 times in ac-LDL group and 1.26 times in ox-LDL group. These results demonstrated that ox-LDL had different effect on the expression and function of ABCA1, ox-LDL might decrease the cholesterol efflux mediated by ABCA1 through other unknown mechanisms.

引用

页码：113 / 116

页数：4