ULTRASTRUCTURAL STUDY ON EXTRAHEPATIC INFECTION OF DUCK HEPATITIS B VIRUS IN DUCKS

<正> Kidney and pancreas tissues from congenitally in-fected Ma ducks with duck hepatitits B virus(DHBV)were examined by immunohistochemical staining tech-nique and electron microscopy.The immunostainingshowed that DHBV antigen was localized in thecytoplasm of tubular epithelial cells of kidney andacinar cells of pancreas of infected ducks.Under elec-tron microscope,we found that complete andincomplete DHBV particles existed in the dilatedcisternae of rough endoplasmic reticulum of both thecells.The size of complete and incomplete virus parti-cles was nearly uniform in both the cells,50-65 nmand 40-50 nm in diameter respectively.Therefore,extrahepatic infection and replication of DHBV weredirectly demonstrated ultrastructurally.