ROLE OF TRANSFORMING GROWTH FACTOR-β IN THE GROWTH INHIBITION OF CULTURED RAT MESANGIAL CELLS IN HIGH GLUCOSE MEDIA

<正>The prenent study was designed to test the hypothesis that the inhibitory effect of elevated glucose levels on mesangial cell growth might be mediated by transforming growth factor β (TGF β). Increased glucose levels inhibited mesangial cell proliferation in a concentration dependent manner. The addition of a rabbit antipncine TGF β1 neutralizing antibody significantly enchanced the cell proliferation in both normal (5.5mM) and high (50mM) glucose media (12% and 30% respectively), and almost completely blocked the growth inhibition induced by high glucose media. TGF β assay demonstrated mesangial cells produced more active than latent TGF β after growth in high glucose media. We conclude that TGF β functions as an autocrine cytokine in mesangial cells growth and the production of TGF β is modulated by high glucose concentration. The growth inhibition induced by high glucose levels may be largely mediated by endogenous TGF β.