Paraquat induces selective dopaminergic nigrostriatal degeneration in aging C57BL/6 mice

被引:0
|
作者
LI Xia YIN Jun CHENG Chunmei SUN Jinlai LI Zheng and WU Yingliang Department of Pharmacology Li X Sun JL and Wu YL Department of Pharmacognosy Yin J Shenyang Pharmaceutical University Shenyang China Department of Pathology Toyama Medical and Pharmaceutical University Toyama Japan Cheng CM [110016 ]
机构
关键词
D O I
暂无
中图分类号
R742.5 [震颤麻痹综合征];
学科分类号
摘要
Background Paraquat (PQ; 1,1’-dimethyl-4,4’-bipyridinium), a widely used herbicide that is structurally similar to the known dopaminergic neurotoxicant MPTP (1-methyl-1,2,3,6-tetrahydropyridine), has been suggested as a potential etiologic factor for the development of Parkinson’s disease (PD). Aging is an accepted risk factor for idiopathic Parkinson’s disease. The aim of this study was to test the hypothesis that paraquat could induce PD-like nigrostriatal dopaminergic degeneration in aging C57BL/6 mice.Methods Senile male C57BL/6 mice were intraperitoneally injected with either saline or PQ at 2-day intervals for a total of 10 doses. Locomotor activity and performance on the pole test were measured 7 days after the last injection and animals were sacrificed one day later. Level of dopamine (DA) and its metabolites levels in the striatum were measured by high-performance liquid chromatography with an electrochemical detector (HPLC-ECD), and numbers of tyrosine hydroxylase (TH) positive neurons were estimated using immunohistochemistry.Results Locomotor activities were significantly decreased and the behavioral performance on the pole test were significantly impaired in the PQ treated group. Level of DA and its metabolites levels in the striatum were declined by 8 days after the last injection. Immunohistochemical analyses showed that PQ was associated with a reduction in numbers of tyrosine hydroxylase positive neurons.Conclusions Long-term repeated exposes to PQ can selectively impair the nigrostriatal dopaminergic system of senile mice, suggesting that PQ could play an important role in the pathogenesis of Parkinson’s disease (PD). Our results also validate a novel model of PD induced by exposure to a toxic environmental agent.
引用
收藏
页码:1357 / 1361
页数:5
相关论文
共 50 条
  • [41] HETEROGENEITY OF BRAIN-REACTIVE AUTOANTIBODIES IN AGING C57BL/6 MICE
    MALLICK, S
    LUEDTKE, RL
    FORSTER, MJ
    LAL, H
    FASEB JOURNAL, 1993, 7 (03): : A55 - A55
  • [42] Early deprivation induces competitive subordinance in C57BL/6 male mice
    Benner, Seico
    Endo, Toshihiro
    Endo, Nozomi
    Kakeyama, Masaki
    Tohyama, Chiharu
    PHYSIOLOGY & BEHAVIOR, 2014, 137 : 42 - 52
  • [43] Spontaneous tumors in aging colonies of C57BL/6J mice
    Schile, Andrew
    Dion, Stephanie
    Imai-Leonard, Denise
    Doty, Rosalinda
    CANCER RESEARCH, 2018, 78 (13)
  • [44] Effects of aging on conventional outflow anatomy and physiology in C57BL/6 mice
    Li, Guorong
    De Ieso, Michael Lucio
    Farsiu, Sina
    Stamer, Daniel
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2020, 61 (07)
  • [45] PROFILING HEMATOPOIETIC STEM CELL AGING IN INDIVIDUAL C57BL/6 MICE
    Dykstra, B. J.
    Olthof, S. G. M.
    Ritsema, M.
    Schreuder, J.
    Gerrits, A.
    Bystrykh, L. V.
    de Haan, G.
    EXPERIMENTAL HEMATOLOGY, 2009, 37 (09) : S25 - S25
  • [46] Change in the distribution and phenotype of subretinal macrophages with aging in C57BL/6 mice
    Aredo, Bogale
    Chen, Xiao
    Zhang, Kaiyan
    Ufret-Vincenty, Rafael
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2013, 54 (15)
  • [47] Neocortical molecular layer heterotopia in substrains of C57BL/6 and C57BL/10 mice
    Lipoff, Danielle M.
    Bhambri, Ankur
    Fokas, Georgia J.
    Sharma, Sanjeev
    Gabel, Lisa A.
    Brumberg, Joshua C.
    Richfield, Eric K.
    Ramos, Raddy L.
    BRAIN RESEARCH, 2011, 1391 : 36 - 43
  • [48] ALUMINUM IN THE ORGANS AND DIET OF AGING C57BL/6J MICE
    MASSIE, HR
    AIELLO, VR
    TUTTLE, RS
    MECHANISMS OF AGEING AND DEVELOPMENT, 1988, 45 (02) : 145 - 156
  • [49] CALCIUM AND CALMODULIN CHANGES WITH AGING IN C57BL/6J MICE
    MASSIE, HR
    AIELLO, VR
    DEWOLFE, LK
    GERONTOLOGY, 1989, 35 (2-3) : 100 - 105
  • [50] DETERMINATION OF THE HISTOCOMPATIBILITY LOCUS INVOLVED IN THE RESISTANCE OF MICE STRAINS C57BL/10-X, C57BL/6-X, AND C57BL/6KS TO C57BL TUMORS
    SNELL, GD
    BORGES, PRF
    JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1953, 14 (03) : 481 - 484