Development, chromosome location and genetic mapping of EST-SSR markers in wheat

被引：0

作者：

CHEN Haimei LI Linzhi WEI Xianyun LI Sishen LEI Tiandong HU Haizhou WANG Honggang ZHANG Xiansheng College of Agronomy Shandong Agricultural UniversityShandong Key Lab of Crop Biology Taian China ^{[271018
]}

机构：

来源：

ChineseScienceBulletin | 2005年 / 20期

关键词：

wheat; EST-SSR; primer; nulli-tetrasome; RIL; genetic map;

D O I：

暂无

中图分类号：

S512.1 [小麦];

学科分类号：

0901 ;

摘要：

A number of 151695 wheat expression sequence tags (ESTs) that originated from GenBank/dbEST from July 14, 2003 to August 24, 2004 were used to search for simple sequence repeats (SSRs) with motif 2―5 bp, and 2038 simple sequence repeats (EST-SSRs), which accounted for 1.34% of EST database, were identified. Based on these SSR sequences, 249 EST-SSR primer pairs and 166 amplified clear bands in various wheat cultivars were designed. These EST-SSR markers can be used as new molecular markers in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 EST-SSR primer pairs and 193 EST-SSR loci were located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic frame- work map previously constructed using recombinant inbred lines.

引用

页码：74 / 82

页数：9

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