Development, chromosome location and genetic mapping of EST-SSR markers in wheat

被引：20
作者：
CHEN Haimei
机构：
来源：
Chinese Science Bulletin | 2005年 / 20期
关键词：
wheat; EST-SSR; primer; nulli-tetrasome; RIL; genetic map;
D O I：
暂无
中图分类号：
S512.1 [小麦];
学科分类号：
0901 ;
摘要：
A number of 151695 wheat expression sequence tags (ESTs) that originated from GenBank/dbEST from July 14, 2003 to August 24, 2004 were used to search for simple sequence repeats (SSRs) with motif 2―5 bp, and 2038 simple sequence repeats (EST-SSRs), which accounted for 1.34% of EST database, were identified. Based on these SSR sequences, 249 EST-SSR primer pairs and 166 amplified clear bands in various wheat cultivars were designed. These EST-SSR markers can be used as new molecular markers in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 EST-SSR primer pairs and 193 EST-SSR loci were located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic frame- work map previously constructed using recombinant inbred lines.
引用
收藏
页码：74 / 82
页数：9
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