SRT1720对瘢痕成纤维细胞的调控作用

被引：0

作者：

严艺文 ^{[1
,2
]}

张婷 ^{[1
]}

郑朝 ^{[1
]}

机构：

[1] 空军军医大学第一附属医院烧伤与皮肤外科

[2] 空军军医大学基础医学院六大队

来源：

中国美容整形外科杂志 | 2024年 / 35卷 / 07期

关键词：

瘢痕成纤维细胞; Ⅰ型胶原蛋白; α-平滑肌肌动蛋白;

D O I：

暂无

中图分类号：

R622 [整形手术学];

学科分类号：

1002 ; 100210 ;

摘要：

目的研究沉默信息调节因子1(silence information regulator 1,SIRT1)的激动剂SRT1720对增生性瘢痕成纤维细胞表型的调控作用。方法自2022年1—6月，空军军医大学第一附属医院烧伤与皮肤外科将瘢痕真皮成纤维细胞（hypertrophic scar fibroblasts,HSF）按随机数字表法分为PBS对照组与SRT1720处理组，样本数9个/组。先分别用等量PBS与终浓度为2μmol/L的SRT1720处理HSF 24 h，然后分别采用实时荧光定量反转录PCR(RT-PCR)法检测HSF中Ⅰ型胶原蛋白（CollagenⅠ）和α-平滑肌肌动蛋白（α-SMA）的m RNA表达水平差异，采用蛋白质印迹法检测HSF中CollagenⅠ和α-SMA的蛋白表达水平差异；采用免疫荧光法检测两组CollagenⅠ和α-SMA在HSF中的胞内表达与定位情况。以上3种细胞实验中每组样本数均为3个。采用GraphPad Prim 8.0进行数据统计分析，组间数据比较采用独立样本t检验，P<0.05为差异有统计学意义。结果 SRT1720处理组HSF中CollagenⅠ与α-SMA的m RNA表达量分别为（0.34±0.06）、（0.43±0.08），均明显低于PBS对照组（1.00±0.12）、（1.00±0.13）,t值分别为8.57、6.79,P<0.01；经SRT1720处理HSF的CollagenⅠ与α-SMA的蛋白表达量（0.70±0.05）、（0.64±0.09），均明显少于PBS对照组的（1.14±0.04）、（1.10±0.05）,t值分别为12.61、7.37,P<0.01。免疫荧光染色显示，CollagenⅠ与α-SMA主要定位在HSF的细胞质，SRT1720处理组CollagenⅠ与α-SMA的表达量较PBS对照组减少。结论 SRT1720能够通过激活SIRT1显著抑制瘢痕成纤维细胞CollagenⅠ与α-SMA表达，从而抑制细胞外基质（extracellular matrix,ECM）合成与成纤维细胞的异常转分化。

引用

页码：395 / 398

页数：4

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