Low-power laser irradiation inhibits arecoline-induced fibrosis: an in vitro study

被引:0
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作者
MeiChun Yeh [1 ]
KerKong Chen [1 ,2 ]
MinHsuan Chiang [1 ,3 ]
ChiaHsin Chen [3 ,4 ,5 ]
PingHo Chen [1 ]
HueyEr Lee [1 ,2 ]
YanHsiung Wang [1 ,3 ]
机构
[1] School of Dentistry,College of Dental Medicine,Kaohsiung Medical University
[2] Department of Dentistry,Kaohsiung Medical University Hospital,Kaohsiung Medical University
[3] Orthopaedic Research Center,College of Medicine,Kaohsiung Medical University
[4] Department of Physical Medicine and Rehabilitation,Kaohsiung Municipal Ta-Tung Hospital,Kaohsiung Medical University
[5] Department of Physical Medicine and Rehabilitation,Kaohsiung Medical University Hospital,Kaohsiung Medical
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R781.5 [口腔粘膜病];
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摘要
Oral submucous fibrosis(OSF) is a potentially malignant disorder that is characterized by a progressive fibrosis in the oral submucosa. Arecoline, an alkaloid compound of the areca nut, is reported to be a major aetiological factor in the development of OSF. Low-power laser irradiation(LPLI) has been reported to be beneficial in fibrosis prevention in different damaged organs.The aim of this study was to investigate the potential therapeutic effects of LPLI on arecoline-induced fibrosis. Arecolinestimulated human gingival fibroblasts(HGFs) were treated with or without LPLI. The expression levels of the fibrotic marker genes alpha-smooth muscle actin(α-SMA) and connective tissue growth factor(CTGF/CCN2) were analysed by quantitative realtime reverse transcription polymerase chain reaction(RT-PCR) and western blots. In addition, the transcriptional activity of CCN2 was further determined by a reporter assay. The results indicated that arecoline increased the messenger RNA and protein expression of CCN2 and α-SMA in HGF. Interestingly, both LPLI and forskolin, an adenylyl cyclase activator, reduced the expression of arecoline-mediated fibrotic marker genes and inhibited the transcriptional activity of CCN2. Moreover, pretreatment with SQ22536, an adenylyl cyclase inhibitor, blocked LPLI's inhibition of the expression of arecoline-mediated fibrotic marker genes. Our data suggest that LPLI may inhibit the expression of arecoline-mediated fibrotic marker genes via the c AMP signalling pathway.
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页码:38 / 42
页数:5
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