Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor

被引:0
作者
LIAO ZhiYong1
2 Capital Institute of Pediatrics
机构
基金
中国国家自然科学基金;
关键词
Chinese sturgeon; GHR; GHBP; mutation; cDNA cloning;
D O I
暂无
中图分类号
S917.4 [水产动物学];
学科分类号
0908 ;
摘要
A full length cDNA encoding the growth hormone receptor (GHR) of Chinese sturgeon was cloned in order to investigate the mechanism of growth hormone in regulating the growth of Chinese sturgeon. The open reading frame of the cloned Chinese sturgeon growth hormone receptor (csGHR) cDNA encodes a trans-membrane protein of 611 amino acids containing all the characteristic motifs of GHR. By sequence alignment, substitutions of amino acid residues highly conserved in other species were identified. Using the CHO cell culture system, the function of csGHR and the biological significance of the amino acid substitution in csGHR were examined. The promoter of serine protease inhibitor 2.1 (Spi2.1) was trans-activated upon stimulation of seabream GH (sbGH) in the csGHR-expressing CHO cells. Furthermore, CHO cells stably expressing csGHR were stimulated to proliferate by sbGH. In agreement with our previous report, Chinese sturgeon growth hormone-binding protein (csGHBP) was detected in the culture medium of CHO cells stably expressing csGHR. Mutation of Asp residue in the ligand binding motif in csGHR to Glu significantly enhanced csGHR’s biological function, whereas mutation of Asp residue to Ala decreased its biological function. The results demonstrated that the cloned csGHR was of full biological function and the csGHBP could be generated through proteolysis of csGHR. These findings might provide new insights into thoroughly understanding the regulatory mechanism of Chinese sturgeon growth.
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页码:911 / 921
页数:11
相关论文
共 12 条
[1]  
Identification and Characterization of GH Receptor and Serum GH-binding Protein in Chinese Sturgeon (Acipenser sinensis)[J]. Zhi-Yong LIAO1,2 and Shang-Quan ZHU2* 1College of Life Sciences, Jiangxi Normal University, Nanchang 330027, China;2Key Laboratory of Proteomics Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China.Acta Biochimica et Biophysica Sinica. 2004(12)
[2]  
Salmon growth hormone receptor: molecular cloning, ligand specificity, and response to fasting[J] . Haruhisa Fukada,Yuichi Ozaki,Andrew L. Pierce,Shinji Adachi,Kohei Yamauchi,Akihiko Hara,Penny Swanson,Walton W. Dickhoff.General and Comparative Endocrinology . 2004 (1)
[3]  
Molecular cloning of a teleost growth hormone receptor and its functional interaction with human growth hormone[J] . Leo T.O Lee,G Nong,Y.H Chan,Dicky L.Y Tse,Christopher H.K Cheng.Gene . 2001 (1)
[4]  
The biology of salmon growth hormone: from daylight to dominance[J] . Fish Physiology and Biochemistry . 1997 (1)
[5]  
Signaling Through the Hematopoietic Cytokine Receptors[J] . J N Ihle,B A Witthuhn,F W Quelle,K Yamamoto,O Silvennoinen.Annual Review of Immunology . 1995
[6]   CIRCULATING GROWTH-HORMONE BINDING-PROTEINS [J].
BAUMANN, G ;
SHAW, MA ;
AMBURN, K .
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, 1994, 17 (01) :67-81
[7]   GROWTH-HORMONE SIGNAL-TRANSDUCTION [J].
MAHARAJAN, P ;
MAHARAJAN, V .
EXPERIENTIA, 1993, 49 (11) :980-987
[8]  
Mutational analysis of the intracellular domain of the human growth hormone receptor .2 Colosi,P,Wong,K,Leong,SR,Wood,WL. J Biol Chem . 1993
[9]  
Alternatively spliced forms in the cytoplasmic domain of the human growth hormone (GH) receptor regulate its ability to generate a soluble GH-binding protein .2 Dastot F,Sobrier ML,Duquesnoy P,Duriez B,Goossens M,Amselem S. Proc. Natl. Acad. Sci. USA . 1996
[10]  
Insulin inhibits growth hormone signaling via the growth hormone receptor/JAK2/STAT5B pathway .2 Ji,S,Guan,R,Frank,SJ,Messina,JL. J Biol Chem . 1999