Pien Tze Huang(片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells

被引：0

作者：

CHEN Xi ^{[1
,2
]}

QI Fei ^{[1
,3
]}

SHEN Aling ^{[1
,3
]}

CHU Jianfeng ^{[1
,3
]}

Thomas Joseph Sferra ^{[4
]}

CHEN Youqin ^{[1
,4
]}

PENG Jun ^{[1
,3
]}

机构：

[1] Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine

[2] Department of Oncology, Fuzhou General Hospital of Nanjing Military Command

[3] Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine

[4] School of Medicine, Case Western Reserve University

来源：

Chinese Journal of Integrative Medicine | 2019年 / 08期

关键词：

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

Objective: To evaluate the effect of Pien Tze Huang(片仔癀, PZH) on breast cancer chemoresistance and related epithelial-mesenchymal transition(EMT) and investigate the underlying mechanisms. Methods: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay was used to determine the cell viability. Adriamycin(ADR) staining observed by fluorescence microscope was performed to detect the accumulation of ADR. Transwell assay was used to analyze the cell migration and invasion. Western-blot was performed to detect the protein expression of related genes. Results: MCF-7/ADR cells were resistant to ADR treatment, and PZH treatment inhibited the viability of MCF-7/ADR cells in a dose-dependent manner. PZH treatment also increased the intercellular accumulation of ADR and down-regulated the expression of ABCG2 and ABCB1 in MCF-7/ADR cells(P<0.05). In addition, PZH treatment inhibited EMT, migration and invasion of MCF-7/ADR cells(P<0.05). Moreover, PZH suppressed activation of transforming growth factor β1(TGF-β) signaling in MCF-7/ADR cells(P<0.05). Conclusion: PZH treatment can effectively overcome chemoresistance via down-regulating ABCG2, ABCB1 and inhibit EMT in ADR resistant human breast cancer cells via suppression of the TGF-β1 pathway.

引用

页码：598 / 603

页数：6

共 18 条

[1]

Pien Tze Huang inhibits the proliferation; and induces the apoptosisand differentiation of colorectal cancer stem cells via suppression of the Notch1pathway[J] Fei Qi;Lihui Wei;Aling Shen;Youqin Chen;Jiumao Lin;Jianfeng Chu;Qiaoyan Cai;Jie Pan;Jun Peng Oncology Reports 2016,

[2]

Pien Tze Huang suppresses VEGF-C-mediated lymphangiogenesis in colorectalcancer[J] Jiumao Lin;Jianyu Feng;Yiyi Jin;Zhaokun Yan;Zijun Lai;Jun Peng Oncology Reports 2016,

[3]

Fluorouracil and dose-dense chemotherapy in adjuvant treatment of patients with early-stage breast cancer: an open-label; 2 × 2 factorial; randomised phase 3 trial[J] Lucia Del Mastro;Sabino De Placido;Paolo Bruzzi;Michele De Laurentiis;Corrado Boni;Giovanna Cavazzini;Antonio Durando;Anna Turletti;Cecilia Nisticò;Enrichetta Valle;Ornella Garrone;Fabio Puglisi;Filippo Montemurro;Sandro Barni;Andrea Ardizzoni;Teresa Gamucci;Giuseppe Colantuoni;Mario Giuliano;Adriano Gravina;Paola Papaldo;Claudia B

[4]

Pien Tze Huang inhibits liver metastasis by targeting TGF-β signalingin an orthotopic model of colorectal cancer[J] Wei Lin;Qunchuan Zhuang;Liangpu Zheng;Zhiyun Cao;Aling Shen;Qiongyu Li;Caixuan Fu;Jianyu Feng;Jun Peng Oncology Reports 2015,

[5]

Global cancer statistics; 2012[J] Lindsey A. Torre;Freddie Bray;Rebecca L. Siegel;Jacques Ferlay;Joannie Lortet‐Tieulent;Ahmedin Jemal CA: A Cancer Journal for Clinicians 2015,

[6]

Reversal of Multidrug Resistance by the Chinese Medicine Yiqi Jianpi Huaji Decoction and the Mechanism of Action in Human Gastric Cancer SGC7901/VCR Cells[J] Wei-Bing Li;Yang Li;Chen Yu;Yong-Ming He;Oliver Micke Evidence-Based Complementary and Alternative Medicine 2015,

[7]

Pien Tze Huang Overcomes Multidrug Resistance and Epithelial-Mesenchymal Transition in Human Colorectal Carcinoma Cells via Suppression of TGF- β Pathway[J] Aling Shen;Hongwei Chen;Youqin Chen;Jiumao Lin;Wei Lin;Liya Liu;Thomas J. Sferra;Jun Peng;Shrikant Anant Evidence-Based Complementary and Alternative Medicine 2014,

[8]

Pien Tze Huang suppresses the stem-like side population in colorectalcancer cells[J] Lihui Wei;Pangyu Chen;Youqin Chen;Aling Shen;Hongwei Chen;Wei Lin;Zhenfeng Hong;Thomas Sferra;Jun Peng Molecular Medicine Reports 2014,

[9]

Pien Tze Huang induces apoptosis in multidrug?resistant U2OS/ADM cellsvia downregulation of Bcl?2; survivin and P-gp and upregulation of Bax[J] Yan Zhang;Qihong Wang;Susheng Niu;Junning Liu;Li Zhang Oncology Reports 2014,

[10]

Inhibition of ovarian cancer cell proliferation by Pien Tze Huang viathe AKT‑mTOR pathway[J] Fan He;Hui‑Ni Wu;Mu‑Yan Cai;Chang‑Peng Li;Xin Zhang;Quan Wan;Shuang‑Bo Tang;Jian‑Ding Cheng Oncology Letters 2014,

← 1 2 →