Effects of stable aqueous fullerene nanocrystal (nC) on Daphnia magna:Evaluation of hop frequency and accumulations under different conditions

We investigated the effects of environmental factors and properties of water-stable crystal fullerene (nC60) on the uptake of nC60 by Daphnia magna based on known accumulation in our laboratory.This study was performed for seven days using different environmental factors including temperature,pH,water hardness,concentration (density of particle),and particle size.Results demonstrated that body burden of C60 increased with time in all experiments.Body burden of C60 increased with increasing concentration and particle size,and uptake of particles >100 nm reached their maximums more quickly than those <100 nm.Under high hardness in aqueous systems with lower pH and high temperature,uptake was higher than those under opposite conditions.Uptake in all batch tests reached balance within five days.Both nC60 properties and environmental factors influenced uptake of nC60 by D.magna in an aqueous system.Additionally,environmental factors may have affected accumulation by changing nC60 properties,which are critical to understand the accumulation of fullerenes in aqueous systems.