INTRACELLULAR PROCESSING AND PRESENTATION OF T-CELL EPITOPES, EXPRESSED BY RECOMBINANT ESCHERICHIA-COLI AND SALMONELLA-TYPHIMURIUM, TO HUMAN T-CELLS

Vaccines based on recombinant attenuated bacteria represent a potentially safe and effective immunization strategy. A carrier system was developed to analyze in vitro whether foreign T cell epitopes, inserted in the outer membrane protein PhoE of Escherichia coli and expressed by recombinant bacteria, are efficiently processed and presented via human leukocyte antigen (HLA) class I and II molecules by bacterial infected human macrophages. A well-defined HLA-B27-restricted cytotoxic T cell (CTL) epitope and an HLA-DR53 restricted T helper (T-h) epitope of the fusion protein of measles virus were genetically inserted in a surface-exposed region of PhoE, and the chimeric proteins were expressed in E. coli and Salmonella typhimurium. Macrophages infected with both recombinant bacteria presented the T-h epitope to the specific CD4(+) T cell clone, but failed to present the CTL epitope to the specific CD8(+) T ceIl clone. Presentation of the T-h epitope by the infected macrophages was inhibited by cytochalasin D, indicating that phagocytic processing of intact bacteria within infected macrophages was essential for antigen presentation via HLA class II. Presentation of the T-h epitope to the CD4(+) T cell clone by infected macrophages was blocked by brefeldin A and cycloheximide, indicating the requirement of nascent HLA class II molecules for presentation. The efficiency of macrophages to process and present the inserted T-h epitope was similar for both the recombinant E. coli and S. typhimurium strains.