TARGETED ONCOGENE ACTIVATION BY SITE-SPECIFIC RECOMBINATION IN TRANSGENIC MICE

被引：516

作者：

LAKSO, M ^{[1
]}

SAUER, B ^{[1
]}

MOSINGER, B ^{[1
]}

LEE, EJ ^{[1
]}

MANNING, RW ^{[1
]}

YU, SH ^{[1
]}

MULDER, KL ^{[1
]}

WESTPHAL, H ^{[1
]}

机构：

[1] DUPONT MERCK PHARMACEUT INC,EXPTL STN E328,WILMINGTON,DE 19880

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 14期

关键词：

CRE/LOX; LENS DEVELOPMENT; SIMIAN VIRUS-40 LARGE TUMOR ANTIGEN;

D O I：

10.1073/pnas.89.14.6232

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An efficient and accurate method for controlled in vivo transgene modulation by site-directed recombination is described. Seven transgenic mouse founder lines were produced carrying the murine lens-specific alpha-A-crystallin promoter and the simian virus 40 large tumor-antigen gene sequence, separated by a 1.3-kilobase-pair Stop sequence that contains elements preventing expression of the large tumor-antigen gene and Cre recombinase recognition sites. Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha-A-crystallin promoter or the human cytomegalovirus promoter. All double-transgenic offspring developed lens tumors. Subsequent analysis confirmed that tumor formation resulted from large tumor-antigen activation via site-specific, Cre-mediated deletion of Stop sequences.

引用

页码：6232 / 6236

页数：5

共 32 条

[1]

[Anonymous], 1986, MANIPULATING MOUSE E

[2] MOLECULAR AND FUNCTIONAL-ORGANIZATION OF YEAST PLASMID-PSR1 [J].

ARAKI, H ;

JEARNPIPATKUL, A ;

TATSUMI, H ;

SAKURAI, T ;

USHIO, K ;

MUTA, T ;

OSHIMA, Y .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 182 (02) :191-203

[3] THE INTEGRASE FAMILY OF SITE-SPECIFIC RECOMBINASES - REGIONAL SIMILARITIES AND GLOBAL DIVERSITY [J].

ARGOS, P ;

LANDY, A ;

ABREMSKI, K ;

EGAN, JB ;

HAGGARDLJUNGQUIST, E ;

HOESS, RH ;

KAHN, ML ;

KALIONIS, B ;

NARAYANA, SVL ;

PIERSON, LS ;

STERNBERG, N ;

LEONG, JM .

EMBO JOURNAL, 1986, 5 (02) :433-440

[4] A NOVEL ROLE FOR SITE-SPECIFIC RECOMBINATION IN MAINTENANCE OF BACTERIAL REPLICONS [J].

AUSTIN, S ;

ZIESE, M ;

STERNBERG, N .

CELL, 1981, 25 (03) :729-736

[5] REPLICATION AND RECOMBINATION FUNCTIONS ASSOCIATED WITH THE YEAST PLASMID, 2-MU CIRCLE [J].

BROACH, JR ;

HICKS, JB .

CELL, 1980, 21 (02) :501-508

[6] MULTIPLEX GENE-REGULATION - A 2-TIERED APPROACH TO TRANSGENE REGULATION IN TRANSGENIC MICE [J].

BYRNE, GW ;

RUDDLE, FH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5473-5477

[7]

CRAIG NL, 1988, ANNU REV GENET, V22, P77

[8] THE FLP RECOMBINASE OF YEAST CATALYZES SITE-SPECIFIC RECOMBINATION IN THE DROSOPHILA GENOME [J].

GOLIC, KG ;

LINDQUIST, S .

CELL, 1989, 59 (03) :499-509

[9] MECHANISM OF STRAND CLEAVAGE AND EXCHANGE IN THE CRE-LOX SITE-SPECIFIC RECOMBINATION SYSTEM [J].

HOESS, RH ;

ABREMSKI, K .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 181 (03) :351-362

[10] DEFECTS IN LENS FIBER DIFFERENTIATION ARE LINKED TO C-MOS OVEREXPRESSION IN TRANSGENIC MICE [J].

KHILLAN, JS ;

OSKARSSON, MK ;

PROPST, F ;

KUWABARA, T ;

VANDEWOUDE, GF ;

WESTPHAL, H .

GENES & DEVELOPMENT, 1987, 1 (10) :1327-1335

← 1 2 3 4 →