MOLECULAR-CLONING AND MAPPING OF PHENOL DEGRADATION GENES FROM BACILLUS-STEAROTHERMOPHILUS FDTP-3 AND THEIR EXPRESSION IN ESCHERICHIA-COLI

被引：39

作者：

DONG, FM ^{[1
]}

WANG, LL ^{[1
]}

WANG, CM ^{[1
]}

CHENG, JP ^{[1
]}

HE, ZQ ^{[1
]}

SHENG, ZJ ^{[1
]}

SHEN, RQ ^{[1
]}

机构：

[1] FUDAN UNIV,INST GENET,SHANGHAI 200433,PEOPLES R CHINA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 08期

关键词：

D O I：

10.1128/AEM.58.8.2531-2535.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two genes of the meta pathway of phenol degradation were cloned from a phenol-utilizing strain of Bacillus stearothermophilus and were mapped by subcloning and by use of a Tn5 insertion mutation. They code for phenol hydroxylase and catechol 2,3-dioxygenase, respectively. The gene encoding catechol 2,3-dioxygenase, which is more thermostable than catechol 2,3-dioxygenase encoded by the other gene, shares rather limited homology with that from Pseudomonas putida.

引用

页码：2531 / 2535

页数：5

共 22 条

[1] THE METABOLISM OF AROMATIC-COMPOUNDS BY THERMOPHILIC BACILLI [J].