THE CYTOPLASMIC PEPTIDOGLYCAN PRECURSOR OF VANCOMYCIN-RESISTANT ENTEROCOCCUS-FAECALIS TERMINATES IN LACTATE

被引：81
作者：
HANDWERGER, S
PUCCI, MJ
VOLK, KJ
LIU, JP
LEE, MS
机构：
[1] BRISTOL MYERS SQUIBB CO,ANTIINFECT MICROBIOL,WALLINGFORD,CT 06492
[2] BRISTOL MYERS SQUIBB CO,ANALYT RES,WALLINGFORD,CT 06492
来源：
JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 18期
关键词：
D O I：
10.1128/jb.174.18.5982-5984.1992
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Vancomycin resistance plasmids in enterococci carry the genes vanH and vanA, which encode enzymes catalyzing, respectively, the reduction of 2-keto acids to 2-D-hydroxy acids and the addition Of D-hydroxy acids to D-alanine. It has therefore been postulated that resistant cells produce peptidoglycan precursors that terminate in the depsipeptide D-alanine-2-D-hydroxy acid rather than the dipeptide D-alanine-D-alanine, thus preventing vancomycin binding (M. Arthur, C. Molinas, T. D. H. Bugg, G. D. Wright, C. T. Walsh, and P. Courvalin, Antimicrob. Agents Chemother. 36:867-869, 1992). In the present work, a cytoplasmic peptidoglycan precursor was isolated from vancomycin-resistant Enterococcus faecalis and analyzed by mass spectrometry, which suggested the structure UDP-N-acetyl-muramyl-L-Ala-D-Glu-L-LyS-D-Ala-D-lactate.
引用
收藏
页码：5982 / 5984
页数：3
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