SELF-ASSEMBLY OF STREPTAVIDIN BISBIOTIN MONOLAYERS AND MULTILAYERS

被引:12
作者
MORGAN, H
TAYLOR, DM
DSILVA, C
FUKUSHIMA, H
机构
[1] Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd LL57 1UT, Dean Street
关键词
D O I
10.1016/0040-6090(92)90400-6
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
It is shown that a solution containing a 2:1 mixture of 1,4-bis(biotinamidomethyl)benzene and streptavidin readily polymerises into rigid, rod-like molecules. The affinity of the protein for the biotin ligand is then exploited in the controlled assembly of a multilayer film of streptavidin approximately 40 nm thick. Surface plasmon resonance measurements confirm that the film is assembled one monolayer at a time.
引用
收藏
页码:773 / 775
页数:3
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