USE OF SUBTRACTIVE TECHNIQUES IN INTERPRETING ONLINE FT-IR SPECTRA OF HPLC COLUMN ELUATES

被引：45

作者：

VIDRINE, DW

机构：

[1] Nicolet Instrument Corporation, Madison, WI, 53711

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1979年 / 17卷 / 08期

关键词：

D O I：

10.1093/chromsci/17.8.477

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flow cell HPLC/FT-IR is especially well suited to absorbance subtraction techniques, because the dilute solutions in constant solvents which are measured eliminate bulk (matrix) effects on subtraction, and because FT-IR allows accurate photometric data to be measured on an extremely precise wavenumber abcissa. Given as practical examples of subtractive applications are the spectroscopic completion of a chromatographically incomplete separation and the critical analysis of a silicone polymer composition versus molecular weight. © 1979, Oxford University Press. All rights reserved.

引用

页码：477 / 482

页数：6

共 10 条

[1]

COFFEY P, 1978, AM LAB, V10, P126

[2]

FLANAGAN M, 1979, 30TH PITTSB C AN CHE

[3] DETERMINATION OF WAVELENGTH SCALE REPEATABILITIES IN COMPUTERIZED SPECTROMETERS [J].