DISTINCT CLASSES OF TRANSCRIPTIONAL ACTIVATING DOMAINS FUNCTION BY DIFFERENT MECHANISMS

被引：353

作者：

TASSET, D ^{[1
]}

TORA, L ^{[1
]}

FROMENTAL, C ^{[1
]}

SCHEER, E ^{[1
]}

CHAMBON, P ^{[1
]}

机构：

[1] FAC MED STRASBOURG,INST CHIM BIOL,INSERM,U184,UNITE BIOL MOLEC & GENIE GENET,F-67085 STRASBOURG,FRANCE

来源：

CELL | 1990年 / 62卷 / 06期

关键词：

D O I：

10.1016/0092-8674(90)90394-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously shown that the two transcriptional activation functions (TAF-1 and TAF-2) of the human estrogen receptor (hER) have synergistic properties different from one another and from those of acidic activating domains (AADs). Here we compare the transcriptional interference/squelching properties of the hER TAFs with those of the AADs of yeast GAL4 and chimeric GAL-VP16 activators. Our results indicate that AADs interact with a factor(s) that, while required for activation by AADs, is not essential for activation by hER TAFs. In contrast, hER TAFs appear to interact with factors indispensable for mediating both their activation function and that of AADs. Thus, different classes of trans-activators may interact with different factors. In addition, the synergistic and transcriptional interference/squelching properties of the two TAFs of the human glucocorticoid receptor (hGR) indicate that both are composed of acidic and nonacidic activation functions. © 1990.

引用

页码：1177 / 1187

页数：11

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