INCREASED RESOLUTION OF LIPOPOLYSACCHARIDES AND LIPOOLIGOSACCHARIDES UTILIZING TRICINE-SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS

被引：300
作者：
LESSE, AJ
CAMPAGNARI, AA
BITTNER, WE
APICELLA, MA
机构：
[1] SUNY BUFFALO, DEPT MED, BUFFALO, NY 14260 USA
[2] SUNY BUFFALO, DEPT MICROBIOL, BUFFALO, NY 14260 USA
[3] SUNY BUFFALO, DEPT PHARMACOL & THERAPEUT, BUFFALO, NY 14260 USA
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 126卷 / 01期
关键词：
Electrophoresis; Lipooligosaccharide; Lipopolysaccharide;
D O I：
10.1016/0022-1759(90)90018-Q
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS. © 1990.
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页码：109 / 117
页数：9
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