INCREASED RESOLUTION OF LIPOPOLYSACCHARIDES AND LIPOOLIGOSACCHARIDES UTILIZING TRICINE-SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS

被引：301

作者：

LESSE, AJ

CAMPAGNARI, AA

BITTNER, WE

APICELLA, MA

机构：

[1] SUNY BUFFALO, DEPT MED, BUFFALO, NY 14260 USA

[2] SUNY BUFFALO, DEPT MICROBIOL, BUFFALO, NY 14260 USA

[3] SUNY BUFFALO, DEPT PHARMACOL & THERAPEUT, BUFFALO, NY 14260 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 126卷 / 01期

关键词：

Electrophoresis; Lipooligosaccharide; Lipopolysaccharide;

D O I：

10.1016/0022-1759(90)90018-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS. © 1990.

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页码：109 / 117

页数：9

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