SOLID-PHASE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR DETECTION OF ANTIBODY TO RUBELLA-VIRUS

被引：9

作者：

GARLAND, SM ^{[1
]}

LOCARNINI, SA ^{[1
]}

GUST, ID ^{[1
]}

机构：

[1] FAIRFIELD HOSP COMMUN DIS,VIRUS LAB,MELBOURNE 3078,VICTORIA,AUSTRALIA

来源：

PATHOLOGY | 1979年 / 11卷 / 03期

关键词：

D O I：

10.3109/00313027909059017

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

An enzyme-linked immunosorbent assay was established for detection of antibodies to rubella virus. In this system commercially available rubella antigen was attached to the wells of polystyrene microtitre plates after which sera were added and incubated to allow the formation of antigen-antibody complexes. The presence of bound antibody was detected by adding anti-human globulin coupled to horseradish peroxidase and visually observing the colour change produced after addition of an appropriate substrate. The test was reproducible and simple to perform and had a similar sensitivity to the widely used haemagglutination inhibition system. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：393 / 399

页数：7

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