Extraction and purification of polyphenol oxidase: A review

被引：60

作者：

Panadare, Dhanashree ^{[1
]}

Rathod, Virendra K. ^{[1
]}

机构：

[1] Inst Chem Technol, Chem Engn Dept, Mumbai 400019, Maharashtra, India

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2018年 / 14卷

基金：

美国国家卫生研究院;

关键词：

Polyphenol oxidase; Extraction; Purification; Characterization;

D O I：

10.1016/j.bcab.2018.03.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyphenol oxidase (PPO) is a copper containing enzyme widely occurred in many plants, animals, bacteria and some fungi. Polyphenol oxidase has wide applications in the field of biosensor formation, food industry, medicine and waste water treatment. Extraction and purification of PPO was studied by many researchers from wide variety of plants and fermentation process using various methods such as salt precipitation, three phase partitioning (TPP), aqueous two phase extraction (ATPE), reverse micellar extraction (RME) and chromatographic techniques. Physio-chemical characterization of the same was performed by the researchers using different techniques. Diversity in the resources of the enzyme also made scientist to study its substrate specificity, optimized activity conditions and inhibitors in detail. Aim of this review is to summarize the detail study regarding extraction and purification of polyphenol oxidase performed by researchers from wide variety of sources.

引用

页码：431 / 437

页数：7

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