AMPEROMETRIC ENZYME ELECTRODE FOR NADP(+) BASED ON A FERRODOXIN-NADP(+) REDUCTASE AND VIOLOGEN-MODIFIED GLASSY-CARBON ELECTRODE

被引：34

作者：

DELACEY, AL ^{[1
]}

BES, MT ^{[1
]}

GOMEZMORENO, C ^{[1
]}

FERNANDEZ, VM ^{[1
]}

机构：

[1] UNIV ZARAGOZA,FAC CIENCIAS,DEPT BIOQUIM & BIOL MOLEC & CELULAR,E-50009 ZARAGOZA,SPAIN

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1995年 / 390卷 / 1-2期

关键词：

BETA-NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; VIOLOGENS; AMPEROMETRIC ENZYME ELECTRODE; GLASSY CARBON ELECTRODE;

D O I：

10.1016/0022-0728(95)03898-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A mediated amperometric enzyme electrode sensitive to NADP(+) was developed by co-immobilization of ferrodoxin-NADP(+) reductase and viologen amino derivatives of different chain length (C-3, C-6 and C-10) on the surface of a glassy carbon electrode. Glutaraldehyde cross-linking in the presence of bovine serum albumin was used for immobilization. Communication between the redox centre of the enzyme and the electrode through the immobilized mediator was proved, and a fast electrical response in the presence of NADP(+) was obtained. The best results were achieved with a medium-sized viologen. The sensitivity and linear response range depended on the polymer thickness and the immobilization procedure affected the stability of the modified electrode.

引用

页码：69 / 76

页数：8

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