REDUCTION OF MONO(ADP-RIBOSYL)ATION OF HISTONES IN RAT TESTIS BY GONADOTROPIN-TESTOSTERONE SYSTEM

被引：7
作者：
KUROKAWA, T
FUJIMURA, Y
TAKAHASHI, K
CHONO, E
ISHIBASHI, S
机构：
[1] Department Physiological Chemistry, Hiroshima University School Medicine, Hiroshima, Minami-ku 734, Kasumi
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 03期
关键词：
D O I：
10.1006/bbrc.1995.2535
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mono(ADP-ribose) synthetase activity in the nuclear fraction of immature rat testis was investigated in reference to the effect of testosterone. When the nuclear fraction was incubated with [P-32] NAD in the presence of nicotinamide, an inhibitor of poly (ADP-ribose) synthetase, some proteins with a molecular mass of 14.4 similar to 21.5 kDa were ADP-ribosylated. In these proteins, H2B and H3 histones were found by sequencing 20 amino acids in the N-terminus. Their incorporations of ADP-ribose moiety were significantly decreased in immature rats at 4 hr after the subcutaneous injection of testosterone. Furthermore, the treatments with LH and FSH of the immature rats reduced mono(ADP-ribosyl)ations of H2B and H3 histones in a time-dependent manner. These results suggest that the testicular mono(ADP-ribose) synthetase activity is under the control of gonadotropin-testosterone system and is possibly related to differentiation of the testis. (C) 1995 Academic Press, Inc.
引用
收藏
页码：808 / 813
页数：6
相关论文
共 22 条
  • [11] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
    LAEMMLI, UK
    [J]. NATURE, 1970, 227 (5259) : 680 - +
  • [12] INHIBITION OF POLY(ADP-RIBOSE) POLYMERASE BY METHYLATED XANTHINES AND CYTOKININS
    LEVI, V
    JACOBSON, EL
    JACOBSON, MK
    [J]. FEBS LETTERS, 1978, 88 (01) : 144 - 146
  • [13] POLY(ADENOSINE DIPHOSPHATE RIBOSE)
    MANDEL, P
    OKAZAKI, H
    NIEDERGANG, C
    [J]. PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, 1982, 27 : 1 - 51
  • [14] DNA-DEPENDENT MONO(ADP-RIBOSYL)ATION OF P-33, AN ACCEPTOR PROTEIN IN HEN LIVER NUCLEI
    MISHIMA, K
    TSUCHIYA, M
    TANIGAWA, Y
    YOSHIMURA, Y
    SHIMOYAMA, M
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 179 (02): : 267 - 273
  • [15] PURIFICATION AND CHARACTERIZATION OF ADP-ROBOSYLARGININE HYDROLASE FROM TURKEY ERYTHROCYTES
    MOSS, J
    TSAI, SC
    ADAMIK, R
    CHEN, HC
    STANLEY, SJ
    [J]. BIOCHEMISTRY, 1988, 27 (15) : 5819 - 5823
  • [16] NOGUCHI J, 1993, J REPROD FERTIL, V97, P433, DOI 10.1530/jrf.0.0970433
  • [17] POLY(ADP-RIBOSYLATION) OF NUCLEAR PROTEINS IN RAT TESTIS CORRELATES WITH ACTIVE SPERMATOGENESIS
    QUESADA, P
    FARINA, B
    JONES, R
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1007 (02) : 167 - 175
  • [18] REGULATION OF THE PHOSPHOINOSITIDE PATHWAY IN CULTURED SERTOLI CELLS FROM IMMATURE RATS - EFFECTS OF FOLLICLE-STIMULATING-HORMONE AND FLUORIDE
    QUIRK, SM
    REICHERT, LE
    [J]. ENDOCRINOLOGY, 1988, 123 (01) : 230 - 237
  • [19] TANIGAWA Y, 1984, J BIOL CHEM, V259, P2022
  • [20] ADP-RIBOSYLATION
    UEDA, K
    HAYAISHI, O
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1985, 54 : 73 - 100
123