INVOLVEMENT OF THE ALPHA-ISOENZYME OF PROTEIN-KINASE-C IN THE GROWTH-INHIBITION INDUCED BY PHORBOL ESTERS IN MH1C1 HEPATOMA-CELLS

被引：30

作者：

PERLETTI, GP ^{[1
]}

SMERALDI, C ^{[1
]}

PORRO, D ^{[1
]}

PICCININI, F ^{[1
]}

机构：

[1] DEPT GEN PHYSIOL & BIOCHEM,I-20133 MILAN,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2848

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MH1C1 rat hepatoma cells express the alpha isoenzyme as the only phorbol-ester sensitive isoform of protein kinase C (PKC). In this cell line, phorbol 12-myristate 13-acetate (PMA) induced a marked, dose-dependent growth inhibition. The administration of the PKC inhibitor staurosporine was able to mimic the effect of the phorbol ester on cell growth in a dose-dependent fashion, whereas the PKC activator arachidonic acid stimulated cell proliferation. Exposure of cells to an antisense oligonucleotide specific for alpha PKC caused a significant impairment of cell growth. These data suggest that the alpha PKC activity is required for proliferation of MH1C1 cells. (C) 1994 Academic Press, Inc.

引用

页码：1589 / 1594

页数：6

共 23 条

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