STOPPING THE CIRCADIAN PACEMAKER WITH INHIBITORS OF PROTEIN-SYNTHESIS

被引：67

作者：

KHALSA, SBS ^{[1
]}

WHITMORE, D ^{[1
]}

BLOCK, GD ^{[1
]}

机构：

[1] UNIV VIRGINIA, NATL SCI FDN, CTR BIOL TIMING, DEPT BIOL, CHARLOTTESVILLE, VA 22901 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 22期

关键词：

RHYTHM; BULLA; MOLLUSK;

D O I：

10.1073/pnas.89.22.10862

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The requirement for protein synthesis in the mechanism of a circadian pacemaker was investigated by using inhibitors of protein synthesis. Continuous treatment of the ocular circadian pacemaker of the mollusc Bulla gouldiana with anisomycin or cycloheximide substantially lengthened (up to 39 and 52 hr, respectively) the free-running period of the rhythm. To determine whether high concentrations of inhibitor could stop the pacemaker, long pulse treatments of various durations (up to 44 hr) were applied and the subsequent phase of the rhythm was assayed. The observed phases of the rhythm after the treatments were a function of the time of the end of the treatment pulse, but only for treatments which spanned subjective dawn. The results provide evidence that protein synthesis is required in a phase-dependent manner for motion of the circadian pacemaker to continue.

引用

页码：10862 / 10866

页数：5

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