DETECTION OF GUANYL CYCLASE IN MAMMALIAN TISSUES

被引：205
作者：
WHITE, AA
AURBACH, GD
机构：
[1] Section on Mineral Metabolism, Metabolic Diseases Branch, National Institute of Arthritis and Metabolic Diseases, Bethesda
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 191卷 / 03期
关键词：
D O I：
10.1016/0005-2744(69)90362-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Guanyl cyclase was assayed by determining the rate of conversion of [α-32P]GTP to cyclic 3′,5′-[32P]GMP. A two-step chromatographic procedure was developed for isolating the product. The enzyme was found in the soluble fraction after homogenizing tissues in 0.3 M sucrose. The reaction showed a pH optimum near 7.6 and was dependent on Mn2+. The relative enzymatic activity for tissues of the rat was lung >> brain = spleen > kidney = liver = heart. © 1969.
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页码：686 / &
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