GAS-PHASE HYDROGEN-DEUTERIUM EXCHANGE AS A MOLECULAR PROBE FOR THE INTERACTION OF METHANOL AND PROTONATED PEPTIDES

被引：113

作者：

GARD, E ^{[1
]}

GREEN, MK ^{[1
]}

BREGAR, J ^{[1
]}

LEBRILLA, CB ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT CHEM,DAVIS,CA 95616

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1994年 / 5卷 / 07期

关键词：

D O I：

10.1016/1044-0305(94)85003-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The gas-phase hydrogen/deuterium (H/D) exchange kinetics of several protonated amino acids and dipeptides under a background pressure of CH3OD were determined in an external source Fourier transform mass spectrometer. H/D exchange reactions occur even when the gas-phase basicity of the compound is significantly larger (> 20 kcal/mol) than methanol. In addition, greater deuterium incorporation is observed for compounds that have multiple sites of similar basicities. A mechanism is proposed that involves a structurally specific intermediate with extensive interaction between the protonated compound and methanol.

引用

页码：623 / 631

页数：9

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