CHARACTERIZATION OF PHE-B-GENE ENCODING CATECHOL 2,3-DIOXYGENASE

被引:1
|
作者
NISHIHARA, T
YAMADA, T
TAKANO, K
OSADA, S
NISHIKAWA, J
IMAGAWA, M
机构
[1] Department of Environmental Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University
关键词
D O I
10.1111/j.1472-765X.1994.tb00938.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The DNA sequence of the phe B gene isolated from a chromosome of the phenol degrading bacterium Pseudomonas putida BH is identical to that of the dmp B gene from the phenol degradative plasmid, pVI15O. Catechol 2,3-dioxygenase encoded by phe B showed similar substrate specificity to that of xyl E. However, Phe B has much smaller K-m values than Xyl E, indicating that Phe B is useful for treatment of low concentrations of catechol derivatives in waste water.
引用
收藏
页码:181 / 183
页数:3
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