SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BOTH THE CLEAVAGE PATTERN AND THE STEREOCHEMICAL OUTCOME OF THE HYDROLYSIS REACTIONS CATALYZED BY VARIOUS GLYCOSIDASES

被引：24
作者：
BRAUN, C
MEINKE, A
ZISER, L
WITHERS, SG
机构：
[1] UNIV BRITISH COLUMBIA,DEPT CHEM,2036 MAIN MALL,VANCOUVER V6T 1Z1,BC,CANADA
[2] UNIV BRITISH COLUMBIA,DEPT MICROBIOL,VANCOUVER V6T 1Z1,BC,CANADA
来源：
ANALYTICAL BIOCHEMISTRY | 1993年 / 212卷 / 01期
关键词：
D O I：
10.1006/abio.1993.1320
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatographic method for the simultaneous determination of both the stereochemical outcome and the cleavage pattern of enzymatic action on unmodified sugar substrates is described. Three different enzymes were investigated by this method. Human pancreatic α-amylase hydrolyzed maltopentaose with retention of anomeric configuration, with the cleavage position being two glucose units from the reducing end. Cellulomonas fimi endoglucanase D hydrolyzed cellopentaose with retention of anomeric configuration and predominantly two glucose units from the reducing end. β-D-Xylosidase from Butyrivibrio fibrisolvens hydrolyzed o-nitrophenyl β-D-xylopyranoside with inversion of anomeric configuration. © 1994 Academic Press, Inc. All rights reserved.
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收藏
页码：259 / 262
页数：4
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