ISOLATION OF A LECTIN FROM THE POLLEN GRAINS OF PETUNIA-HYBRIDA

被引：2
|
作者：
BAJAJ, M
SHIVANNA, KR
机构：
来源：
ACTA BOTANICA NEERLANDICA | 1991年 / 40卷 / 02期
关键词：
LECTIN; PETUNIA; POLLEN GRAINS; SELF-INCOMPATIBILITY;
D O I：
10.1111/j.1438-8677.1991.tb01520.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A lectin was isolated and partially characterized from the pollen grains of Petunia hybrida. The lectin, isolated by gel filtration on Sephacryl S-200 and affinity chromatography on Concanavalin A-agarose, was found to have a molecular weight (approximate) of 105 000 D. The lectin agglutinated rat, mouse, sheep and human erythrocytes and required Ca2+ and Mg2+ for haemagglutinating activity. Sugar specificity of the lectin could not be elucidated. Acid phosphatase activity was associated with haemagglutinating activity throughout the purification procedure.
引用
收藏
页码：125 / 132
页数：8
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