Effect of Laser Priming on accumulation of Free Proline in Spring Durum Wheat (Triticum turgidum L.) under Salinity Stress

Experiments were conducted during 2012 in a greenhouse of College of Abouraihan, University of Tehran, Iran. In this study the effects of salt stress and laser priming on proline content of durum wheat (Triticum turgidum L.) was carried out in a factorial experimental based on a randomizely complete block design (RCBD) with three replications. Seeds from two cultivars salt sensitive and salt tolerance of Durum wheat (Triticum turgidum L.) were exposed to neodymium-doped yttrium aluminum garnet (Nd-Yag) laser irradiation (75 mW cm(-2), radiated for 12 min). Salinity treatments carried out in four levels (Control, 70, 140 and 210 mM) via sodium chloride. The sampling from first leaves was carried out on four stages of growth and their proline content was measured. The result showed that free proline content in leaves increased significantly by increasing of NaCl concentration and salt tolerant variety accumulate more Proline than sensitive variety. Also proline content significantly increased with irradiation by laser beam in salinity condotion. These results indicate that the low power continuous wave Nd-Yag laser light seed treatment has considerable biological effects on plant metabolism. This seed treatment technique can be potentially employed to enhance agricultural productivity.