GEL-ELECTROPHORESIS OF FLUORESCENT LABELED CYANOGEN-BROMIDE CLEAVAGE PRODUCTS AT THE SUB-MICROGRAM LEVEL

被引：17
作者：
CHENKIANG, S
STEIN, S
UDENFRIEND, S
机构：
[1] ROCHE INST MOLEC BIOL, NUTLEY, NJ 07110 USA
[2] ROCKEFELLER UNIV, DEPT MOLEC CELL BIOL, NEW YORK, NY 10021 USA
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 01期
关键词：
D O I：
10.1016/0003-2697(79)90194-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple method is described for visualizing submicrogram amounts of the CNBr cleavage products of proteins on sodium dodecyl sulfate-polyacrylamide gels. The peptide fragment mixture is conjugated with the fluorogenic reagent 2-methoxy-2,4-diphenyl-3-(2H)-furanone prior to electrophoresis. The fluorescnet peptide bands are visible under UV light, avoiding the need for fixation and staining. The determination of the structural homology of 2 immunologically related proteins [rat skin prolyl hydroxylase and a cross reacting protein] is presented to illustrate this methodology.
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页码：122 / 126
页数：5
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