DECREASE OF MELTING TEMPERATURE AND SINGLE STRAND SCISSION OF DNA BY BLEOMYCIN IN PRESENCE OF HYDROGEN PEROXIDE

被引：70

作者：

NAGAI, K

SUZUKI, H

TANAKA, N

UMEZAWA, H

机构：

[1] Institute of Applied Microbiology, University of Tokyo, Tokyo, Bunkyo-ku

来源：

JOURNAL OF ANTIBIOTICS | 1969年 / 22卷 / 12期

关键词：

D O I：

10.7164/antibiotics.22.624

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Effect of bleomycin and H2O2 on DNA was investigated. Tm of salmon sperm DNA decreased from 76°C to 64°C and that of Escherichia colt DNA from 81°C to 65.5°C in the presence of 100 μM of H2O2 and 4μg/ml of bleomycin. The change of Tm was more marked when the concentration of H2O2 or bleomycin was increased. The single strand scission of DNA was shown when the DNA was incubated at 37°C with 100/μlM of H2O2and 40 μg/ml of bleomycin. It proceeded as the time of incubation was prolonged. The reaction of H2O2 at 100μm on DNA was apparent only in the presence of bleomycin. © 1969, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.

引用

页码：624 / &

共 17 条

[1] SINGLE-STRAND SCISSIONS INDUCED IN CIRCULAR AND LINEAR LAMBDA DNA BY PRESENCE OF DITHIOTHREITOL AND OTHER REDUCING AGENTS [J].