MICELLAR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF SERUM BILIRUBIN SPECIES WITH DIRECT SAMPLE INJECTION

A rapid reversed-phase high performance Liquid chromatographic method is described using a C-8 column and a mobile phase containing sodium dodecyl sulfate (SDS) as a modifier to separate the four bilirubin species present in serum: unconjugated, monoconjugated, diconjugated and biliprotein. The results show that using SDS as a mobile phase modifier improves separation efficiency and increases sample solubility. This simple HPLC procedure allows direct sample injection and makes it possible to quantitatively determine bilirubin species in biological fluids.