PROLIFERATION OF CHICK PRIMORDIAL GERM-CELLS CULTURED ON STROMA CELLS FROM THE GERMINAL RIDGE

被引：34
作者：
CHANG, IK
TAJIMA, A
CHIKAMUNE, T
OHNO, T
机构：
[1] INST PHYS & CHEM RES,RIKEN CELL BANK,TSUKUBA,IBARAKI 305,JAPAN
[2] UNIV TSUKUBA,INST AGR & FORESTRY,TSUKUBA,IBARAKI 305,JAPAN
来源：
CELL BIOLOGY INTERNATIONAL | 1995年 / 19卷 / 02期
关键词：
D O I：
10.1006/cbir.1995.1055
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Fluorescent reagent-labelled PGCs isolated from the blood of 2-day-old chick embryos were cultured on stroma cells derived from 5-day-old germinal ridge in Medium 199 supplemented with 10% FBS, human IGF-1, bovine FGF-b, and murine LIF. In 7 experiments, the number of MCs increased by an average of 4.8 fold in 4 days. Intrinsic PGCs in the 5-day embryonic germinal ridge were observed loosely attached to the stroma cells, and they also increased 3.8 fold during culture for 4 days. These results indicate the possibility of applying this culture method to the production of transgenic chickens.
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页码：143 / 149
页数：7
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