EVALUATION OF INTRASPECIES GENETIC-VARIATION WITHIN THE 16S RIBOSOMAL-RNA GENE OF MYCOPLASMA-HOMINIS AND DETECTION BY POLYMERASE CHAIN-REACTION

被引：92
作者：
BLANCHARD, A
YANEZ, A
DYBVIG, K
WATSON, HL
GRIFFITHS, G
CASSELL, GH
机构：
[1] UNIV ALABAMA,DEPT MICROBIOL,VOLKER HALL,ROOM 507,BIRMINGHAM,AL 35294
[2] INST PASTEUR,F-75724 PARIS,FRANCE
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 05期
关键词：
D O I：
10.1128/JCM.31.5.1358-1361.1993
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Mycoplasma hominis is a heterogeneous species with DNA-DNA hybridization values ranging from 51 to 100%. We report here the sequencing of the 16S rRNA gene of a strain (183) that greatly differs from the type strain (PG21) of this species. Comparison of 16S rDNA sequences from these two strains showed limited differences, indicating that the two strains belong to the same rRNA species complex. Using these nucleotide sequence data, we established a rapid method for the detection of M. hominis by using polymerase chain reaction. This method was shown to be sensitive and specific when tested with reference strains and clinical isolates.
引用
收藏
页码：1358 / 1361
页数：4
相关论文
共 23 条
  • [1] ANDERSEN H, 1987, J GEN MICROBIOL, V133, P181
  • [2] DETECTION AND IDENTIFICATION OF MYCOPLASMAS BY AMPLIFICATION OF RDNA
    BLANCHARD, A
    GAUTIER, M
    MAYAU, V
    [J]. FEMS MICROBIOLOGY LETTERS, 1991, 81 (01) : 37 - 42
  • [3] BROWN MB, 1983, SEX TRANSM DIS, V10, P247
  • [4] PERINATAL MYCOPLASMAL INFECTIONS
    CASSELL, GH
    WAITES, KB
    CROUSE, DT
    [J]. CLINICS IN PERINATOLOGY, 1991, 18 (02) : 241 - 262
  • [5] CASSELL GH, 1989, INFECT DIS, P632
  • [6] CASSELL GH, 1990, ATLAS SEXUALLY TRANS
  • [7] CHRISTIANSEN C, 1987, ISRAEL J MED SCI, V23, P591
  • [8] CHRISTIANSEN G, 1987, ISRAEL J MED SCI, V23, P595
  • [9] HETEROGENEITY AMONG MYCOPLASMA-HOMINIS STRAINS AS DETECTED BY PROBES CONTAINING PARTS OF RIBOSOMAL RIBONUCLEIC-ACID GENES
    CHRISTIANSEN, G
    ANDERSEN, H
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1988, 38 (01): : 108 - 115
  • [10] HOW CLOSE IS CLOSE - 16S RIBOSOMAL-RNA SEQUENCE IDENTITY MAY NOT BE SUFFICIENT TO GUARANTEE SPECIES IDENTITY
    FOX, GE
    WISOTZKEY, JD
    JURTSHUK, P
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (01): : 166 - 170
123