PHYSICAL LOCALIZATION OF YEAST CYS3, A GENE WHOSE PRODUCT RESEMBLES THE RAT GAMMA-CYSTATHIONASE AND ESCHERICHIA-COLI CYSTATHIONINE GAMMA-SYNTHASE ENZYMES

被引：13
作者：
BARTON, AB
KABACK, DB
CLARK, MW
KENG, T
OUELLETTE, BFF
STORMS, RK
ZENG, B
ZHONG, WW
FORTIN, N
DELANEY, S
BUSSEY, H
机构：
[1] MCGILL UNIV,DEPT BIOL,YEAST CHROMOSOME I PROJECT,MONTREAL H3A 1B1,QUEBEC,CANADA
[2] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT MICROBIOL & MOLEC GENET,NEWARK,NJ 07103
[3] CONCORDIA UNIV,DEPT BIOL,MONTREAL H3G 1M8,QUEBEC,CANADA
[4] MCGILL UNIV,DEPT MICROBIOL & IMMUNOL,MONTREAL H3A 2B4,QUEBEC,CANADA
来源：
YEAST | 1993年 / 9卷 / 04期
关键词：
SACCHAROMYCES-CEREVISIAE; CHROMOSOME-I; SULFUR ASSIMILATION; CYS3;
D O I：
10.1002/yea.320090406
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned, sequenced and physically mapped the CYS3 gene of Saccharomyces cerevisiae. This gene can complement the cys3-1 allele, and disruptions at this locus lead to cysteine auxotrophy. The predicted CYS3 product is closely related (46% identical) to the rat cystathionine gamma-lyase (Erickson et al., 1990), but differs in lacking cysteine residues. These results provide further evidence that the S288C strain of yeast resembles mammals in synthesizing cysteine solely via a trans-sulfuration pathway. The CYS3 product was found to have strong homology to three other enzymes involved in cysteine metabolism: the Escherichia coli metB and metC products and the S. cerevisiae MET25 gene product. The trans-sulfuration enzymes appear to form a diverged family and carry out related functions from bacteria to mammals.
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收藏
页码：363 / 369
页数：7
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