CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF HUMAN ANTITHROMBIN-III

被引：9

作者：

SCHREUDER, H

DEBOER, B

PRONK, S

HOL, W

DIJKEMA, R

MULDERS, J

THEUNISSEN, H

机构：

[1] UNIV GRONINGEN,BIOSON RES INST,9747 AG GRONINGEN,NETHERLANDS

[2] AKZO PHARMA DIV,ORGANON SCI DEV GRP,5340 BH OSS,NETHERLANDS

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 229卷 / 01期

关键词：

ANTITHROMBIN-III; CRYSTALLIZATION; SERPINS; BLOOD COAGULATION; 3-DIMENSIONAL STRUCTURE;

D O I：

10.1006/jmbi.1993.1024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human antithrombin III has been crystallized from 18 to 21% (w/v) polyethylene glycol 4000 at pH 7.15. The spacegroup is P21 with cell parameters a = 89.8 AÅ, b = 100.8 AÅ, c = 70.0 AÅ and β = 106°. The diffraction limit is 3.2 AÅ. The asymmetric unit contains two protein molecules. Analysis of dissolved crystals for biological activity and by gel electrophoresis suggests that one protein molecule in the asymmetric unit is intact, while the other is cleaved. © 1993 Academic Press, Inc.

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页码：249 / 250

页数：2

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