STIMULATION OF ANTIMYCOBACTERIAL ACTIVITY IN MOUSE PERITONEAL-MACROPHAGES BY PRIMING WITH TREHALOSE DIMYCOLATE (TDM)

We examined the potential of two bacterial immunomodulators, trehalose dimycolate (TDM) and lipopolysaccharide (LPS), to stimulate the capacity of mouse peritoneal macrophages to control the growth of the intracellular bacterium, Mycobacterium tuberculosis BCG. Macrophages were obtained from mice innately susceptible (Bcg(s)) or resistant (Bcg(r)) to BCG infection. In all mouse strains tested (Bcg(r) and Bcg(s)), with the exception of BALB/c (Bcg(s)), TDM was sufficient to elicit macrophages with strong antimycobacterial activity in vitro. In BALB/c mice, the induction of anti-BCG activity required two signals, TDM and LPS, given in sequence. Our data suggest that additional gene(s), besides the Bcg locus, control macrophage resistance to BCG.