CONFOCAL FLUORESCENT MICROSCOPY WITH A FINITE-SIZED CIRCULAR DETECTOR

被引：53
|
作者：
GU, M
SHEPPARD, CJR
机构：
[1] Department of Physical Optics, University of Sydney, NSW
来源：
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION | 1992年 / 9卷 / 01期
关键词：
D O I：
10.1364/JOSAA.9.000151
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
For a confocal fluorescent microscope with a finite-sized circular detector, the three-dimensional optical transfer function (OTF) for a thick object has been developed without the use of Stockseth's approximation. The results show that the OTF has negative values when the radius of the detector exceeds certain magnitudes. The two-dimensional OTF derived from the three-dimensional OTF is also given.
引用
收藏
页码：151 / 153
页数：3
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