MEASUREMENT OF MALONDIALDEHYDE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION

被引：273

作者：

YOUNG, IS

TRIMBLE, ER

机构：

[1] Department of Clinical Biochemistry, Queen's University of Belfast, Belfast BT12 6BA, Grosvenor Road

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1991年 / 28卷

关键词：

LIPID PEROXIDATION; THIOBARBITURIC ACID; FREE RADICALS;

D O I：

10.1177/000456329102800514

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The thiobarbituric acid test for malondialdehyde is widely used as an indicator of lipid peroxidation and free radical activity. We describe a simple and sensitive high performance liquid chromatographic (HPLC) method for measuring the malondialdehyde-thiobarbituric acid adduct using fluorimetric detection. Extraction into organic solvent or other complex sample preparation is not required and the assay is sensitive to < 0.05-mu-mol/L. This method could easily be applied to any setting where a measure of lipid peroxidation is required.

引用

页码：504 / 508

页数：5

共 21 条

[1]

ANDREWS FJ, 1989, IRON IMMUNITY CANCER, P145

[2] DETERMINATION OF MALONALDEHYDE IN BIOLOGICAL-MATERIALS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].