PREPARATIVE SEPARATION OF PYROGENS FROM PROTEINS BY ISOELECTRIC-FOCUSING USING A MULTICOMPARTMENT ELECTROLYZER WITH IMMOBILINE MEMBRANES

被引：9
|
作者：
LUCAS, J [1 ]
FAUPEL, M [1 ]
GOECKING, C [1 ]
机构：
[1] CIBA GEIGY AG,BIOTECHNOL & EXPLORATORY RES SERV,CH-4002 BASEL,SWITZERLAND
来源：
ELECTROPHORESIS | 1990年 / 11卷 / 11期
关键词：
D O I：
10.1002/elps.1150111120
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Due to the nature of lipopolysaccharide endotoxin structures of bacterial pyrogens, their removal from solutions containing therapeutic proteins is often a problem in the pharmaceutical industry. In this report we describe the application of electromotive force to dislodge lipopolysaccharide endotoxins from proteins. This was performed by employing a multicompartment electrolyzer fitted with Immobiline membranes of specified pIs. A thousand‐fold reduction of endotoxin could be achieved in the model test system described. This contribution describes the use of a new recycling isoelectric focusing approach without the use of carrier ampholytes. Copyright © 1990 VCH Verlagsgesellschaft mbH
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收藏
页码：981 / 982
页数：2
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