PREPARATIVE LIQUID-CHROMATOGRAPHY - SAMPLE VOLUME OVERLOAD

The maximal injection volume equation has been developed from the well-known expression for the output profile resulting from large sample input volume in elution chromatography. The validity of this equation is examined experimentally. In order to quantify the maximal sample load under volume overload conditions, the maximal injection concentration in the maximal injection volume is studied in terms of column dimensions and chromatographic parameters. A sampling valve connected with a solute sprinkler is used with a split-stream technique and is compared with other injection modes with respect to band spreading and column loadability. © 1979.