CYCLODEXTRIN-FACILITATED BIOCONVERSION OF 17-BETA-ESTRADIOL BY A PHENOLOXIDASE FROM MUCUNA-PRURIENS CELL-CULTURES

被引：37

作者：

WOERDENBAG, HJ ^{[1
]}

PRAS, N ^{[1
]}

FRIJLINK, HW ^{[1
]}

LERK, CF ^{[1
]}

MALINGRE, TM ^{[1
]}

机构：

[1] UNIV GRONINGEN,CTR PHARM,DEPT PHARMACOGNOSY,9713 AW GRONINGEN,NETHERLANDS

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 05期

关键词：

17β-estradiol; bioconversion; Fabaceae; Mucuna pruriens; ortho-hydroxylation; phenoloxidase; β-cyclodextrin;

D O I：

10.1016/0031-9422(90)80119-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

After complexation with β-cyclodextrin, the phenolic steroid 17β-estradiol could be ortho-hydroxylated into a catechol, mainly 4-hydroxyestradiol, by a phenoloxidase from in vitro grown cells of Mucuna pruriens. By complexation with β-cyclodextrin the solubility of the steroid increased from almost insoluble to 660 μM. The bioconversion efficiency after 72 hr increased in the following order: freely suspended cells (0%), immobilized cells (1%), cell homogenate (6%), phenoloxidase preparation (40%). Mushroom tyrosinase converted 17β-estradiol, as a complex with β-cyclodextrin, solely into 2-hydroxyestradiol, with a maximal yield of 30% after 6-8 hr. Uncomplexed 17β-estradiol was not converted at all in any of these systems. © 1990.

引用

页码：1551 / 1554

页数：4

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