PURIFICATION OF THE INHIBITOR OF THE 3'-5' CYCLIC-AMP PHOSPHODIESTERASE OF DICTYOSTELIUM-DISCOIDEUM BY AFFINITY CHROMATOGRAPHY

被引：8
作者：
DICOU, E
BRACHET, P
机构：
[1] Institut Pasteur Département, Biologie Moléculaire Unité de Différenciation Cellulaire, 75724 Paris Cedex 15
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 04期
关键词：
D O I：
10.1016/0006-291X(79)91180-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method is described to purify in a single step the inhibitor of the 3′-5′ cyclic AMP phosphodiesterase of D. discoideum by affinity chromatography to phosphodiesterase immobilized to Sepharose. Biologically active, pure inhibitor is eluted with 5M salt at high yields. Application of this technique to the study of mutants which overproduce or are deficient in the synthesis of the inhibitor is presented. © 1979.
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页码：1321 / 1327
页数：7
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