GAS CHROMATOGRAPHY OF CATECHOLAMINE METABOLITES USING ELECTRON CAPTURE DETECTION AND MASS SPECTROMETRY

被引：127
作者：
ANGGARD, E
SEDVALL, G
机构：
[1] Department of Pharmacology, Karolinska Institutet, Stockholm
来源：
ANALYTICAL CHEMISTRY | 1969年 / 41卷 / 10期
关键词：
D O I：
10.1021/ac60279a042
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Conditions were studied for the gas chromatography of normetanephrine, metanephrine, 3-methoxytyra-mine, 3-methoxy-4-hydroxyphenyl ethylene glycol, vanillylmandelic acid, and homovanillic acid with electron capture (EC) detection and mass spectrometry. Pentafluoropropionates (PFP) and heptafluorobutyrates were more stable and had higher EC-responses than the corresponding trifluoroacetates. These derivatives were also used to separate the amines on XE-60 and the deaminated metabolites on OV-17. The linear range with the63Ni EC-detector was 0,02-0.8 ng for the PFP-derivatives. Mass spectra were obtained from the perfluoroacylated, trimethylsilylated, and acetylated metabolites. © 1969, American Chemical Society. All rights reserved.
引用
收藏
页码：1250 / &
相关论文
共 25 条
[1]   METABOLISM OF EPINEPHRINE AND OTHER SYMPATHOMIMETIC AMINES [J].
AXELROD, J .
PHYSIOLOGICAL REVIEWS, 1959, 39 (04) :751-776
[2]   STUDIES OF TRIFLUOROACETIC ACID .2. PREPARATION AND PROPERTIES OF SOME TRIFLUOROACETYL ESTERS [J].
BOURNE, EJ ;
TATLOW, CEM ;
TATLOW, JC .
JOURNAL OF THE CHEMICAL SOCIETY, 1950, (MAY) :1367-1369
[3]   SEPARATION AND IDENTIFICATION OF DERIVATIVES OF BIOLOGIC AMINES BY GAS-LIQUID CHROMATOGRAPHY [J].
CAPELLA, P ;
HORNING, EC .
ANALYTICAL CHEMISTRY, 1966, 38 (02) :316-&
[4]   ELECTRON CAPTURE PROPERTIES OF HALOGENATED AMINE DERIVATIVES [J].
CLARKE, DD ;
WILK, S ;
GITLOW, SE .
JOURNAL OF GAS CHROMATOGRAPHY, 1966, 4 (08) :310-&
[5]   GAS CHROMATOGRAPHIC DETERMINATION OF DOPAMINE AT NANOGRAM LEVEL [J].
CLARKE, DD ;
WILK, S ;
GITLOW, SE ;
FRANKLIN, MJ .
JOURNAL OF GAS CHROMATOGRAPHY, 1967, 5 (06) :307-+
[6]   A GAS-LIQUID-CHROMATOGRAPHIC PROCEDURE FOR SEPARATING A WIDE RANGE OF METABOLITES OCCURRING IN URINE OR TISSUE EXTRACTS [J].
DALGLIESH, CE ;
HORNING, EC ;
HORNING, MG ;
KNOX, KL ;
YARGER, K .
BIOCHEMICAL JOURNAL, 1966, 101 (03) :792-+
[7]   DETERMINATION OF URINARY 3-METHOXYTYRAMINE NORMETANEPHRINE AND METANPHRINE IN PHEOCHROMOCYTOMA AND NEUROBLASTOMA BY GAS CHROMATOGRAPHY [J].
GREER, M ;
SPRINKLE, TJ ;
WILLIAMS, CM .
CLINICA CHIMICA ACTA, 1968, 21 (02) :247-&
[8]   MASS FRAGMENTOGRAPHY - IDENTIFICATION OF CHLORPROMAZINE AND ITS METABOLITIES IN HUMAN BLOOD BY A NEW METHOD [J].
HAMMAR, CG ;
HOLMSTEDT, B ;
RYHAGE, R .
ANALYTICAL BIOCHEMISTRY, 1968, 25 (1-3) :532-+
[9]   TRYPTAMINE-RELATED AMINES + CATECHOLAMINES [J].
HORNING, EC ;
BROOKS, CJW ;
KNOX, KL ;
VANDENHEUVEL, WJA ;
HOLMSTEDT, B ;
HORNING, MG .
ANALYTICAL CHEMISTRY, 1964, 36 (08) :1546-&
[10]  
HORNING HG, 1967, BIOCHIM BIOPHYS ACTA, V148, P597
123