DIRECT CHIRAL SEPARATIONS BY CAPILLARY ELECTROPHORESIS USING CAPILLARIES PACKED WITH AN ALPHA(1)-ACID GLYCOPROTEIN CHIRAL STATIONARY-PHASE

被引：211

作者：

LI, S ^{[1
]}

LLOYD, DK ^{[1
]}

机构：

[1] MCGILL UNIV,DEPT ONCOL,MCINTYRE MED BLDG,3655 DRUMMOND,ROOM 717,MONTREAL H3G 1Y6,QUEBEC,CANADA

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 24期

关键词：

D O I：

10.1021/ac00072a023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fused-silica capillaries of 50-mum internal diameter were packed with an alpha1-acid glycoprotein chiral stationary phase. The properties of electroosmosis in these packed capillaries were studied by investigating the influence of field strength, pH, and solvent composition on the velocity of electroosmotic flow. Direct enantiomeric separation by capillary electrochromatography was investigated with the use of these packed capillaries. Chiral resolution was achieved for the enantiomers of benzoin, hexobarbital, pentobarbital, ifosfamide, cyclophosphamide, disopyramide, metoprolol, oxprenolol, alprenolol, and propranolol. The effects of pH, electrolyte concentration, and concentration of organic solvents in the mobile phase on the retention and the enantioselectivity were studied.

引用

页码：3684 / 3690

页数：7

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