NITROGEN OXIDE-INDUCED AUTOPROTECTION IN ISOLATED RAT HEPATOCYTES

被引：183
作者：
KIM, YM
BERGONIA, H
LANCASTER, JR
机构：
[1] UNIV PITTSBURGH,SCH MED,DEPT SURG,PITTSBURGH,PA 15261
[2] LOUISIANA STATE UNIV,MED CTR,DEPT PHYSIOL,NEW ORLEANS,LA 70112
[3] LOUISIANA STATE UNIV,MED CTR,DEPT MED,NEW ORLEANS,LA 70112
来源：
FEBS LETTERS | 1995年 / 374卷 / 02期
关键词：
NITRIC OXIDE; NITROGEN OXIDE; RAT HEPATOCYTE; REACTIVE OXYGEN SPECIES;
D O I：
10.1016/0014-5793(95)01115-U
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pretreatment of rat hepatocytes with low-dose nitrogen oxide (addition of SNAP in vitro or induction of nitric oxide synthase in vitro or in vivo) imparts resistance to killing and decrease in aconitase and mitochondrial electron transfer from a second exposure to a higher dose of SNAP. Induction of this resistance is prevented by cycloheximide, indicating upregulation of protective protein(s). Ferritin levels are increased as are nonheme iron-NO EPR signals. Tin-protoporphyrin (SnPP) prevents protection, suggesting involvement of hsp32 (heme oxygenase) and/or guanylyl cyclase (GC). Cross-resistance to H2O2 killing is also observed, which is also prevented by cycloheximide and SnPP. Thus, hepatocytes possess inducible protective mechanisms against nitrogen oxide and reactive oxygen toxicity.
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页码：228 / 232
页数：5
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